Normalize Every Microbe:
Your input range just got a lot more forgiving
One Workflow, Zero Compromise: Robust Microbial Library Prep Across Any Input with AutoNorm™
Fixed-cycle PCR doesn't care that your samples aren't all the same — but your sequencing data does. When paired with seqWell's ExpressPlex™ Library Prep, iconPCR with AutoNorm expands the effective normalization range from ~5-fold all the way to 40-fold by dynamically adjusting amplification cycles per well in real time. Whether your samples span a 100-fold input range or a mixed-GC microbial panel, every library hits its optimal endpoint — no individual QC required before pooling.
- Expands ExpressPlex normalization range from ~5-fold to up to 40-fold with AutoNorm adaptive amplification
- Handles 100-fold DNA input variation (2–200 ng) and mixed GC-content microbes in the same plate
- Balanced read representation across all samples — more data from every pooled sequencing run
- Library structure preserved: consistent insert size and no added GC bias
- One-step tagmentation + amplification entirely on the icon96 — pool and sequence, done
