Automated Normalization of Small RNA Libraries with iconPCR™ and NEXTFLEX™ Chemistry

On Demand Webinar

Join n6’s Yann Jouvenot and Revvity’s Pedro Echave for a practical look at how AutoNorm™ technology helps simplify small RNA library prep. Using data generated with the NEXTFLEX Small RNA-Seq Kit v4, they show how iconPCR can automatically adjust PCR cycling for variable RNA inputs, reduce over-amplification, and improve yield consistency across tissues with very different microRNA content.
The result is a more efficient workflow with less guesswork, better reproducibility, and no detectable bias in RNA composition.

What You'll Learn

• How AutoNorm uses real-time fluorescence to stop amplification at the right endpoint for each sample.
• Why fixed-cycle PCR can create uneven yields and over-amplification in small RNA workflows.
• How iconPCR™ and NEXTFLEX v4 work together to simplify normalization at the PCR step.
• What the validation data showed for brain, muscle, and placenta samples at 1 ng and 10 ng inputs.
• Why this workflow can improve reproducibility while reducing hands-on optimization.