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Application Note

Metagenomics Sequencing

Learn how to optimize your next microbiome sequencing project by minimizing bias in 16S library prep

Application Note  Metagenomics

Bring Every Microbe to Light: 
16S rRNA Sequencing, Unfiltered

Unlock the full story in your microbiome research with iconPCR™ and AutoNorm™—delivering next-level 16S sequencing for every sample, every run. Skip the biases and dropped species of using traditional PCR for 16S library prep: thanks to real-time, per-well control, you’ll capture truer community profiles, reduce chimeras by up to 4x, and reveal more unique amplicon sequence variants (ASVs)—including the rare and elusive. Whether you’re running short or full-length 16S seq, you get balanced, deeply resolved 16S libraries and hands-off pooling—no matter the input or sample source. Discover how easy it is to leave artifacts and normalization headaches behind, so your data reflects the real microbiome diversity in every tube.

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Kitchen Sink Data Hightlight

Curious what happens when you throw the whole ecosystem at iconPCR? Check out our “kitchen sink” experiment, where we threw every matrix (kitchen sink swab coming soon, maybe) in a single 96-well run—skin, soil, fecal, mouth, controls—each one normalized, amplified, and balanced perfectly, no extra hands-on required. The result: beautifully resolved, well-balanced reads from the most complex mixtures science can throw at you.