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Application Note

FFPE & Degraded DNA

ffpe app note

Rescue Every Read: 
Don’t sequence them like you did 

Rescue Every Read: FFPE & Degraded Sample Success, Stress-Free

The phrase “challenging sample” just got taken off your worry list. iconPCR with AutoNorm transforms FFPE (Formalin-Fixed, Paraffin-Embedded) and other degraded specimens from data risk to NGS goldmine. Where traditional protocols can over- or under-cycle, leading to dropouts, high duplication rates, or ghost reads, iconPCR uses real-time fluorescence to ensure every library gets just the right push—no matter DIN or input variability. Rescue poor-quality prep with high-confidence amplification, optimal yield, and balanced normalization into your capture or sequencing pool. Run your ultra-precious, low-yield samples plus the “easy” ones side-by-side in one smart, streamlined workflow—with reliable results every time and no extra cost on hands or reagents.

  • Dramatically reduces dropouts and reruns— even from DNA hanging on by a thread.
  • Maximizes data from every precious, rare sample — boosting coverage and minimizing wasted reads.
  • All in a workflow that lets you finally say “yes” to even the most intimidating material in the archive.
normalization of degraded samples graph